The Identifying Factor Of Azotobacter

The Identifying Factor Of Azotobacter It is very simple to disregard the dirt microorganisms that are so vital to the soundness of the vegetation of a biological system. When talking about soil microorganisms, this order can be additionally separated into three subcategories: parasites, protista and microbes, with microscopic organisms making up the biggest part of the microorganisms (Boyle et al., 2007, Pelczar et al. 1993). These dirt microorganisms assume a focal job to the biogeochemical cycling of supplements, for example, Nitrogen, Phosphorus and Sulfur; components which are inconvenient for the development and life of life forms (Bastida et al., 2007). Notwithstanding their job in supplement cycling, soil microorganisms to a great extent add to soil structures by separating and breaking down natural issues, and are likewise a significant food hotspot for different life forms, for example, night crawlers and amoebae (Bastida et al., 2007). Thinking about the effect of soil microorganisms on the earth and biological system, it is anything but difficult to perceive how soil microorganisms additionally sway human way of life and economy. As of late, a surge of examination has been directed on the significance of microorganisms on farming, with interests in the capacity of nitrogen fixing microscopic organisms to fill in for nitrogenous manures (Cakmacki et al. 2006). This lab concentrated on the bacterial bit of soil microorganisms, explicitly, the seclusion and recognizable proof of a solitary bacterium from a nearby soil test utilizing various aseptic research center strategies and Bergeys Manual of Systemic Bacteriology (1984). Materials and Methods The accompanying strategies were taken from the Biology 203 Lab Manual (Robertson 2008). Two soil tests were taken, the first from backwoods soil and the second, a coarse woody garbage, from fertilizer. These two examples were sequentially weakened to make arrangements from 10E-2 to 10E-7, and from each example pour plates, inclines, streak plates and stocks were immunized utilizing aseptic strategy. The state morphologies were watched and recorded and each plate was specified. From these examples 4 microbes were picked and subcultured onto streak plates and inclinations. In the wake of getting ready and Gram Staining the slides the cell morphologies of the microbes were watched and noted. The states were again subcultured onto streak plates and tried for the capacity to hydrolyze starch by including Iodine. The states were likewise refined into Sulfide, Indole and Motility (SIM) deeps to test for the existences of the Sulfur cycle and motility. Likewise Peptone stocks were vaccinate d and the way of life were tried for ammonification. Ammonium sulfate stocks and nitrite stocks were vaccinated and nitrification was tried for too denitrification was tried for by immunizing nitrate stocks. Oxygen consuming breath was affirmed when catalase tests were completed by adding H2O2 to an example of bacterium. At long last societies were independently presented to every one of a few distinct temperatures, salinities and levels of pH so as to decide their ideal ecological conditions. A solitary settlement of microbes was picked to be recognized: microscopic organisms 1 from soil test 2. Results The settlement morphology can be depicted as a flickering dark white shading with a level and unpredictable shape. The development was smooth and delicate. The distance across of the province approximated 15mm. Under 1000x amplification it was uncovered that the cells were bacilli, singlet and had a measurement of around 2î ¼m. The cells recolored Gram negative. Table 1: Summary of Results for Unidentified Bacterium 1 of Soil Sample 2 Test Result Starch Hydrolysis Positive H2S decrease Negative Motility Negative Oxygen consuming or Anaerobic Oxygen consuming Ammonification Positive Denitrification (NO3-to NO2-) Negative Nitrification (NH3/NH4+ to NO2-) Positive Nitrification (NH3/NH4+ to NO3-) Positive Catalase Positive Ideal temperature 22Â °C Ideal pH 5 Ideal salt fixation 0% The consequences of the rest of the tests biochemical and natural are summed up by Table 1. It was finished up dependent on the Iodine and starch response that this bacterium hydrolyzed starch as a wellspring of Carbon. The Sulfur cycle didn't happen as there was no dark hasten from the mix of Iron and hydrogen sulfide found in the SIM deeps. The SIM deeps revealed that these microbes were non-motile, becoming just on the wound line. The proteins in the peptone stock were corrupted to smelling salts meaning that this bacterium is an ammonifer. Nitrification was likewise affirmed with the microbes oxidizing the NH3 and NH4+ in the stocks to NO2-and NO3. Denitrification nonetheless, didn't happen; NO3 was not diminished. The expansion of H2O2 prompted rising as it responded with catalase present in the cells. Ideal ecological conditions were seen as 22Â °C, pH of 5 and 0% saltiness. Between the temperatures of 4, 15, 22 and 54Â °C, development was most grounded at 22Â °C, at that po int 15 and most fragile at 37 and 4Â °C . Development at pH was just somewhat more grounded than at pH 7 yet considerably more grounded than at pH 3 and 9 (allude to table 2). Development in saltiness was best at 0% and diminished with .05%, 2% and 5% individually. Table 2: Growth of bacterium 1 at different pH dependent on absorbance levels at 580nm pH Bacterial Growth 3 .227 5 .692 7 .510 9 .147 Conversation Every one of these means helped in the conceivable distinguishing proof of the bacterium as Azobacteraceae Azotobacter a genera of microorganisms found in soil, water and roots (Bergeys Manual, 1984). Because of the more slender layer of peptidoglycan encompassed by a phospholipid external film rather than a thick outer layer of peptidoglycan this bacterium recolored Gram negative (Prescot, Harley and Klein 2005). Of indispensable significance for recognizable proof was the nearness of catalase, a compound present in oxygen consuming microscopic organisms that separates the poisonous result of electron transport: H2O2 (Wang et al. 2008). The pursuit to recognize Bacterium 1 started with these two wide standards: Gram negative and oxygen consuming breath. This specific bacterium was non-motile, the bacterium became uniquely along the wound line in the SIM profound instead of spreading all through the medium. This ended up being a significant factor while distinguishing as it as Azotob acter which contains both motile and non-motile microorganisms (Bergeys Manual, 1984). These three characteristics alone pointed toward Azotobacter; the biochemical and natural tests served to affirm that Bacterium 1 was to be sure Azotobacter by coordinating the attributes of this specific genera to the bacterium. Nitrification was a typical trademark between the two and was affirmed to happen in Bacterium 1 when the smelling salts stock was oxidized to nitrite and nitrite was oxidized to nitrate (Bergeys Manual, 1984). Denitrification be that as it may, didn't happen the nitrate was left flawless and un-diminished. Ammonification, the breakdown of nitrogen containing mixes to alkali, was likewise a typical quality of Bacterium 1 and Azotobacter (Bergeys Manual, 1984, Roberts, 2008). In spite of the fact that the test for motility in the SIM profound was certain, the sulfur cycle test in the SIM had a negative outcome. The sulfur containing mixes were not decreased by the bacterium to deliver H2S and, this outcome even as a negative, was a significant factor in distinguishing proof on the grounds that Azotobacter additionally doesn't diminish sulfur (Bergeys Manual, 1984, Roberts, 2008). Bergeys Manual (1984) characterizes Azotobacter as a heterotroph and, comparably Bacterium 1 was distinguis hed as a heterotroph when Iodine was added to the streak plate containing starch and no shading change happened in the territory under and around the state. This meant that the bacterium separating and utilizing the starch. At last, the ideal ecological states of both Bacterium 1 and Azotobactera were seen as fundamentally the same as. The ideal conditions were expressed as: pH of 4.8 8.5, temperature of 15 37ËÅ ¡C and low saltiness (Bergeys Manual, 1984). Bacterium 1 had fundamentally the same as ecological states of: pH of 5, 22ËÅ ¡C and saltiness of 0% NaCl. Thinking about the nitrifying and ammonifiying characteristics of the Azotobacter, this bacterium assumes a significant job in the nitrogen cycle by separating proteins and changing over the nitrogen into a structure that would then be able to be utilized by different life forms (Butenschoen, Marhan and Scheu, 2007, Cakmakci et al. 2006). Azotobacter, as one of the more typical nitrifying soil organisms, is known to deliver a lot of usable Nitrogen, and in this manner is firmly connected to plant development and wellbeing (Cakmakci et al. 2006, Prescot, Harley and Klein 2005). Curiously, albeit many nitrifying creatures hold a cooperative relationship with plant roots, giving Nitrogen in return to supplements, Azotobacter, specifically, doesn't (Prescot, Harley and Klein 2005). Azotobacter additionally completes starch hydrolysis, and in this way helps in the decay of natural issue in soil and the mineralization procedure (Smith and Smith, 2001). The recognizable proof of Bacterium 1 as Azobacteraceae Azotobacter isn't clear and a few different tests would have required so as to demonstrate this announcement as obvious. One test which would have been useful would have been a test for blister arrangement; a key attribute of the Azotobacter (Bergeys Manual, 1984, Prescot, Harley and Klein 2005). The Azotobacter are not rhizobacteria and along these lines it would have been proper if there were some method of watching the microbes in their regular living space. What's more, the tests that were done had constraints to the measure of data that could have been gathered from the outcomes. The nitrification, ammonification, and denitrification tests depended on a basic shading change, and there was no chance to get of telling the procedure by which these capacities, if present, happened, nor were the tests explicit to the grouping of the mixes present. These tests and strategies could likewise have been exposed to mistake, for instan ce, measu

What Is Modernism Essay Example | Topics and Well Written Essays - 3250 words

What Is Modernism - Essay Example The article What Is Modernism breaks down innovation development. Each change is guided by novel principals for this case innovation included a break from indigenous conventions and consistent movement. Pioneers praised knowledge, radical reasoning and innovativeness on the grounds that these were the capacities of humanity moreover, they didn't fall hostage of indigenous cutoff points. Authenticity period included workmanship that painted the subject precisely as it might have been, for instance, Edouard Manet painted a French concubine ‘Olympia’ striking, reckless and unashamed. This was the first occasion when that specialists parted from conventional measures. Impressionism followed where specialists painted impressions of the genuine subjects. What they depicted is a differentiation between what they see and that which the cerebrum forms, drawing out the effect of logical information on craftsmanship. From this period, the ‘isms’ poured forward, which i ncluded post-impressionism, fauvism and cubism. Craftsmen used each chance to loosen up from ordinarily held lessons and methods. Innovation had well eminent and productive specialists like Salvador Dali, Pablo Picasso, George Seurat and Marcel Duchamp only yet to make reference to a couple. Innovation included almost the entire of twentieth century craftsmanship; it is presently straightforward why innovation is a huge purpose of conversation by workmanship pundits and students of history. All things considered, seeing innovation from an alternate edge, it’s not tied in with painting. There are different teaches as Music, theory, writing and figure, the rundown is long.

How to Write a College Book Report Paper

The most effective method to Write a College Book Report Paper The most effective method to Write a College Book Report Paper Book reports are characterized as enlightening reports that conscious on a particular book from a target perspective. Understudies have commonly mistaken book reports for book surveys since they are not aware of the distinctions that exist between the two. Book reports sum up the work while book surveys gives assessment of works close by. Essentially, book reports give records of the significant characters, plot, proposal, and the primary ideas of the work. A book report is a short rundown that ranges from 275 to 500 words. Understudies who are given book report assignments should be educated regarding the essential data concerning the book. These incorporate the book title, the writer, the distributer and spot of production, just as number of pages. This data is vital since it is joined into the report. Before starting on a book report, it is judicious to decide the class of the book. Composing a fiction book report expects one to be perceptive of the primary characters, the setting , and the setting of activity. Genuine book reports include stressing the primary thoughts introduced in the book. Understudies experience book report writing in their school level of learning. This is trying since educators expect work of a higher caliber than on the secondary school level. The most effective method to compose a school book report is a requesting issue for rookies understudies. For one to compose a school book report viably, one must peruse the particular book being referred to. Perusing a long work may appear to be a repetitive undertaking particularly if the book doesn’t intrigue the peruser. What's more, understudy essayists must see each idea that is introduced in the book. The main perusing is typically lacking regardless of whether the peruser really centers around the substance of the book. Taking notes on the significant focuses just as page quantities of where such ideas are outlined in the book helps one in recollecting the book’s plot just as composing a viable report. The understudy may likewise discover examining proficient studies of the book supportive in understanding the plot, portrayal, and use of artistic gadgets or methods. In any case, it is imperative to limit one’s research with the goal that the understudy peruses just the investigates that premium the understudy the most. Scholarly book reports require the essayist to follow the rules gave by the teacher. This guarantees the understudy essayist remains inside the parameters of the report venture. For a true to life book, the understudy should concentrate on points and issues that the understudy most appreciated or discovered particularly interesting. On a similar note, the understudy should offer individual understanding concerning the advancement of the plot or portrayal or the work of artistic gadgets. School papers are dubious to compose since their premise of evaluation is centered around data from the book that the understudy essayist presents. In rundown, remain concentrated on the motivation behind the paper. It isn't to give a scrutinize. Or maybe, it is to give an abstract of what the book contains. Likewise with any composing task, building up a blueprint for direction as one peruses can be useful in figuring out what one needs to state. Compose thoughts in a reasonable and intelligent style. What's more, one ought to consistently set aside some effort to amend and to edit one’s paper before submitting it for an evaluation. Undergrads can get proficient school book report help on the web, at composing administration. Let your book report paper composed by top of the line essayists.

5 Paragraph Format Essay Where To Look For A Sample

Natural Therapies Institute Where to Get a Good Example of 5 Paragraph Essay A five paragraph essay is always a change. This is because the student has to express their thoughts and information in a short space. Where to get a good example of 5 paragraph essay, can greatly help the student. By showing them how to develop ideas and means to present them. Textbooks and Handbooks Web Sites Libraries Textbooks and Handbooks Most students should have a personal library that has samples of a five paragraph essay. Textbooks and the writing handbooks, as well as the style guides will have sample essays in them. These examples can be used to show the student how to write a 5 paragraph essay. They will also have instructions on writing them to use with the examples. These books need to be in every student’s personal library. Three books that every student should have in their personal library are; English Textbook Writer’s Handbook At least one Style Guide (MLA) The reason is these will help the student in more than just the samples they will contain. They will also help the student in writing their essays. Web Sites Web sites can vary in quality, but Student help sites, and College/ University sites are the best. This is because these sites qualify the material they allow for them. College and University sites usually use a student that had an exemplary essay. The school or instructor wants to use this an an example. Student help sites use qualified writers to create sample essays for students to use, and then check them for errors. Some DIY sites are also critical of what they put up. But these sites need to be checked before using, the writer’s submission page is the best to look at. If they tell the writer all work is critiqued before posting, then they should be good. Libraries Both the public and School libraries have many resources for these examples. Books on writing, as well as some media can be found on them. There are many anthologies of essays, and most libraries will have a good number of them. These will have anything from five paragraph essays to one to two thousand word essays. They will often have an assortment of writing books. These will have explanations and samples of different essays, including five paragraph essays. Some School Libraries may even have samples in their passion for students to look over.Expert admission essay writing service - get your essays written by professional application essay writer.

Identify The Identities Of Two Unknown Bacteria A Mixed...

Abstract: The purpose of this project was to identify the identities of two unknown bacteria in a mixed broth culture by using several separation methods. To separate the organisms, a four-way streak plate technique was used to isolate the two unknown bacteria into separate visible colonies. Then after each colony were clearly isolated; the two unknowns were processed through Gram staining test to determine the Gram stain and morphology. After Gram staining, a carbohydrate test was performed on each unknown to determine if it had glucose, sucrose, or lactose fermentation. The results of the sugar test help determining which biochemical test should be performed next. The Gram positive organism was tested through a carbohydrate fermentation test, then further tested to confirm its identity through an indole and catalase test. The Gram negative organism was tested through carbohydrate fermentation test, then further tested to confirmed its identity through an indole, and TSIA test. Afte r running four biochemical tests, the results conclude that the Gram positive unknown was Staphylococcus aureus. S. aureus was identified based on the fermentation results of the glucose test, negative indole test, and a positive catalase production. S. aureus is a Gram positive circular shaped bacterium that is very common in the U.S and is normally found in the nose, respiratory tract, and on the skin. This bacterium is usually the most common cause of infections after injury or surgery.Show MoreRelatedIdentifying And Identify Two Unknown Bacteria From A Mixed Culture1737 Words   |  7 Pagescorrect disease diagnosis in patients and for proper treatment. Knowing the correct identity and characteristics of microorganism is crucial when disease outbreaks occur in populations, also knowing how humans can benefit from microorganisms is important; many can be used in making certain foods or antibiotics. The purpose of this study project was to carefully isolate and identify two unknown bacteria from a mixed culture. The ability to properly evaluate biochemical test results is also necessary forRead MoreMicrobiology Labs6972 Words   |  28 PagesLab Report Name: ____________________ Section: ___________________ EXPERIMENT 1 TITLE: Observing Bacteria and Blood OBJECTIVE: To gain functional knowledge of microscope operations through practical applications of a microscope in the observation of bacteria and blood. PROCEDURES: Using the microscope, an oil immersion lens and observing Bacteria Cultures in Yogurt . Preparing a Blood Slide and observing Blood: After reviewing the section of the manual as instructed I

Descriptive Essay On Beauty - 850 Words

Beauty. Every person has a different standard of it. The hill country is my place of beauty and peace. Green trees and grass. Flowers blooming and butterflies everywhere. Birds singing their melodies and deer roaming peacefully. The sparkling sun causing everything around us to sparkle. Lampasas, Texas is a little town many people know nothing of. However, it’s the closest place to Heaven on this earth. My family is from there. My great-grandma has a house on the outside of Lampasas. The beauty of that land is surreal. It is the perfect picture of the country that is blessed with a little country home, many acres, cows, a creek filled to the brim and flowing ever so peacefully, the crickets chirping, and every now and then a car may pass†¦show more content†¦There was no way on earth I was going to be able to climb The Bluff and not die. I eventually sucked it up, with the help of my grandfather, and started my trek up The Bluff. The obstacles I faced were ginormous, at least for the younger me. Daddy Long Legs climbing all round us, housed in the nooks and crannies we needed to climb up the bluff. With every hand and foot hole held a home for them. Our hands and feet went in clean and came out with two or three Daddy Long Legs on them. Rocks, sticks, and uncomfortable surfaces scraping up and slicing our wet, wrinkly hands. Grass burrs were a gift from Satan himself. Lo and behold, they were absolutely everywhere. All the while detecting a little voice behind me, not paying much attention to it. The heavy breaths were drowning any other sound out. We were inching our way up the bluff, step by step. My little, dark, normally a brunette but a blonde due to the sun, brother was in front of me by quite a bit. I was struggling and barely staying up there. With cuts all over me, spiders petrifying me, and not being able to pull myself up, I started to get discouraged and tried to go down. I mumbled to myself, â€Å"Well, you were never the athletic one anyways. They are strong and good at this, but I can’t do it.† Then I heard the little voice a little clearer and then very clearly. The voice was telling me, â€Å"You can do this†, â€Å"Don’t give up†, and â€Å"You are stronger than this trial youShow MoreRelatedDescriptive Essay About Beauty947 Words   |  4 PagesI think our minds are obsessed with beauty. We envy those we cannot be. We strive to have this picture perfect life, or something close to it. When I was younger, I was never out going or the kid that talked all the time. That just wasn’t me. I never thought of myself as beautiful. I thought I was pretty, but in my mind, to be truly beautiful, one must possess other characteristics beyond the physical realm. 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Descriptive essay is purposely created so readers can readily imagine its particular subject matter. It focuses on the five senses which are sight, smell, touch, sound and taste. 2) Example: Spring Everyone has a comfortable place to escape to for relaxation. They go there when they need to beRead MoreAnalysis Of The Santa Ana And Linda Thomass Brush Fire1500 Words   |  6 PagesJoan Didion in her essay, â€Å"The Santa Ana† and Linda Thomas in her essay, â€Å"Brush Fire† describes the Santa Ana in two opposing stands with similar moves. Didions purpose in writing her essay for the Santa Ana is to inform her readers. She informs them about the Santa Ana, the effect the winds have on human behavior, and how they have to live with the Santa Ana. Thomas writes her essay to engage readers on the Santa Ana’s effect on brushes. 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In the introduction of Orwell’s essay, he explains that he knew from a very young age that he was meant to be a writer, but that he chose to abandon that idea. In doing so, he felt that he was â€Å"outraging his true nature†. This phraseRead MoreThe Basis Of Hume s Theory1398 Words   |  6 PagesThe basis of Hume’s essay is in regards to aesthetics, he offers his definition of the â€Å"standard of taste† from the standpoint of even the best critics. Throughout the essay, Hume reaches his conclusion by â€Å"both his characterization of the appreciative response and by his particular way of drawing the distinction between the subjective and the objective,† (Carroll). From the beginning of the essay, it is noticed that Hume’s theory is representative of a paradox. 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Managing Consumer Markets for Accountancy - MyAssignmenthelp.

Question: Discuss about theManaging Consumer Markets for Accountancy. Answer: Introduction VENDOR app is the name of the application, the primary objective of the application is to connect buyers and sellers on an ideal and classified platform to negotiate and do the transaction by creating a secure and a comprehensive market platform. With an expected 200 companies and about 500 individual to utilize the application on their commercial activities, for the first two months of the application launch. With the number expected to increase over time. The application will be a free to download application either for Android or iOS users (Wen, et al. (2014). The Benefits of the Application The application will mostly target sales people in the mainstream industries like care sales, computers, and electronics, real estate business, land agencies, phone and much more. With a personal or individual platform also available. In which the users will post their products according to the categorization provided by the application. Location, time and price will all be a categorizing aspect of making help buyers have easy access to the products on the selling list(Bowerman, DeLorme, (2014). The app is unique from other online classified application as it utilizes the visual aspect on a deferent level. This is achieved, through the execution of a video call ability, where the interested party is able to video call the seller direct. Also, there will be a free video posting for the various products sold where the seller can record a video and post it along with the details of the item, with is acting as a supplement to the picture post of the product. This will performance as an advertisement platform where the video can incorporate audio aspects. The app will also consist of the electronic money platform where the transactions can take place within the network instead of other deferent avenues. That are prone to Insecurity and lack of assured privacy in terms of personal financial information. With all the features programmed in a secure way to avoid and mischiefs activates as the background staff members will also be monitoring the processes on a 24-hour basis. The application will be a free to download the app, with compatibility with on the android and windows operating system. With a modified software for the computers also available (Drew, (2013). The company will be taking a 30% cut of every transaction made on the platform as a pay and also 5% for every money withdrawal done using the vendor app electronic wallet. The app is expected to have monthly maintenance as an inclusive way for any updates in terms of adding new features, improving on security measures and developing the application according to the users requirements or preference in case of any. The Applications main Goal to Address The primary problem that the app intends to eliminate is the fraud in the online market. This is because in recent times more and more issues of cyber security are on the rise. With the problem lowering online business. For instance, most people have been conned by individuals posting fake items, and after negotiating the item price with the interested party and some amount of money transferred by the name down payment, the seller happens to close all communication avenues. Or in the case where after proper negotiations for the product and both parties meet for the transaction processes. The seller turns to a thief, to which they extort the money and take away the sale item too. Through high-tech firewalls which will be updated according to the changes and improvements in the technology industry (Christopher, et al. (1991). A highly secured database that will be linked to some of the security databases, for example, the credit burial so as to avoid any blacklisted persons from the ap plication platform. The application will also require credible documents from all users so as to monitor their transactions and as well as keep reliable records of each user. With an active anti-virus fitted to verify all the posts from the public. Another measure is that money will be posted by the buyer if they are content with the pricing of the item on sale. The after full delivery and efficient verification of the product. The customer will authorize the managing team to transfer the agreed amount to the sellers account. This move will help to increase, validation practice and appreciation of the products bought from the app and also enhance the safety of the transactions held (Sheth, Parvatlyar, (1995). User Guide The application will be available on the play store site for all interested users to download for free. After installation, the user will specify their main activity based on either; they are a company or individual. This will be followed the specification of either; the user is only a seller a buyer or both of them. This is because the interface between the vendor and the customer are not the same regarding the arrangements of the icons and their accessibility which depends on how quickly the user utilizes them, for instance, the take a video icon will not be visible for the buyer only user. Sellers Once the application is downloaded, installed and filled in the specifications. The seller will include their physical location the company or individual name, picture, followed by their contacts and a description of the kind of business they deal with, which will be accompanied by an advertising message of why they are the best to transact with them. That sort of information is what will act as their profile on the buyer's interface. Buyer The user will also specify their name, address, and contacts. After which the will verify their e-wallet information. From this step, they will be able to see all the current posts on their gadgets. And if any specific requirements are needed, the app provides a text box where they can write their interested items. Once the posted products are displayed, which will vary depending on the specifications on the search icon. In terms of location, price, and condition, the buyer will click the on the negotiation icon, which will link both the seller and buyer. And if they agree on their negotiation the buyer will release the amount to the application manager platform. The Application Monitoring Team After the negotiations are done, and every detail monitored, the managing team will delete the item from the selling platform and post it on the buyers negotiation platform. After which they will wait for an agreement notice so as to release the money to the seller. And if there will be any disagreement the both of them will send a disappointment notice where the item will be displayed back to the selling list and the money forwarded back to the buyer. At such a case the management team will investigate on whose flat it was. Then if validated the accused person will have a compensation fee of 5% of the item price to the other party. This kind of measures and penalties are implemented to increase effectiveness and credibility of the application and its users at large (Schmitz, et al. (2016). Conclusion The VENDOR app will act as a middle man in all the transactions carried out on the application platform. The app will provide what other classified applications dont manage. That is the security and a surety of the transactions to the users. By this, the app is expected to receive positive remarks from the target market, so as to help achieve the objective number of users. With future implementations an actual goal, such as, providing a mobile money transfer network that is not restricted by country or region boundaries as the current market provides. Reference Drew, J. (2013). How to develop and publish a mobile app. Journal of Accountancy, 215(2), and 24. Sheth, J. N., Parvatlyar, A. (1995). Relationship marketing in consumer markets: antecedents and consequences. Journal of the Academy of Marketing Science, 23(4), 255-271. Christopher, M., Payne, A., Ballantyne, D. (1991). Relationship marketing: bringing quality customer service and marketing together. Wen, D. M. H., Chang, D. J. W., Lin, Y. T., Liang, C. W., Yang, S. Y. (2014, June). Gamification design for increasing customer purchase intention in a mobile marketing campaign app. In International Conference on HCI in Business (pp. 440-448). Springer International Publishing. Schmitz, C., Bartsch, S., Meyer, A. (2016). Mobile App Usage and its Implications for Service ManagementEmpirical Findings from German Public Transport. Procedia-Social and Behavioral Sciences, 224, 230-237. Bowerman, K., DeLorme, D. E. (2014). Boaters perceptions of a mobile app for a marine conservation social marketing campaign. Social Marketing Quarterly, 20(1), 47-65.